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Sequence analysis of a 101-kilobase plasmid required for agar degradation by a Microscilla isolate.

ABSTRACT: An agar-degrading marine bacterium identified as a Microscilla species was isolated from coastal California marine sediment. This organism harbored a single 101-kb circular DNA plasmid designated pSD15. The complete nucleotide sequence of pSD15 was obtained, and sequence analysis indicated a number of genes putatively encoding a variety of enzymes involved in polysaccharide utilization. The most striking feature was the occurrence of five putative agarase genes. Loss of the plasmid, which occurred at a surprisingly high frequency, was associated with loss of agarase activity, supporting the sequence analysis results.

SUBMITTER: Zhong Z

PROVIDER: S-EPMC93371 | biostudies-literature | 2001 Dec

REPOSITORIES: biostudies-literature

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Sequence analysis of a 101-kilobase plasmid required for agar degradation by a Microscilla isolate.

Zhong Z Z Toukdarian A A Helinski D D Knauf V V Sykes S S Wilkinson J E JE O'Bryne C C Shea T T DeLoughery C C Caspi R R

Applied and environmental microbiology 20011201 12

An agar-degrading marine bacterium identified as a Microscilla species was isolated from coastal California marine sediment. This organism harbored a single 101-kb circular DNA plasmid designated pSD15. The complete nucleotide sequence of pSD15 was obtained, and sequence analysis indicated a number of genes putatively encoding a variety of enzymes involved in polysaccharide utilization. The most striking feature was the occurrence of five putative agarase genes. Loss of the plasmid, which occurr ...[more]

PMID: 11722934

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