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Ca²⁺ and aminoguanidine on γ-aminobutyric acid accumulation in germinating soybean under hypoxia-NaCl stress.

ABSTRACT: Gamma-aminobutyric acid (GABA), a nonproteinous amino acid with some benefits on human health, is synthesized by GABA-shunt and the polyamine degradation pathway in plants. The regulation of Ca²⁺ and aminoguanidine on GABA accumulation in germinating soybean (Glycine max L.) under hypoxia-NaCl stress was investigated in this study. Exogenous Ca²⁺ increased GABA content significantly by enhancing glutamate decarboxylase gene expression and its activity. Addition of ethylene glycol tetra-acetic acid into the culture solution reduced GABA content greatly due to the inhibition of glutamate decarboxylase activity. Aminoguanidine reduced over 85% of diamine oxidase activity, and 33.28% and 36.35% of GABA content in cotyledon and embryo, respectively. Under hypoxia-NaCl stress, the polyamine degradation pathway contributed 31.61-39.43% of the GABA formation in germinating soybean.

SUBMITTER: Yang R

PROVIDER: S-EPMC9351771 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Ca2+ and aminoguanidine on γ-aminobutyric acid accumulation in germinating soybean under hypoxia-NaCl stress.

Yang Runqiang R Guo Yuanxin Y Wang Shufang S Gu Zhenxin Z

Journal of food and drug analysis 20141106 2

Gamma-aminobutyric acid (GABA), a nonproteinous amino acid with some benefits on human health, is synthesized by GABA-shunt and the polyamine degradation pathway in plants. The regulation of Ca2+ and aminoguanidine on GABA accumulation in germinating soybean (Glycine max L.) under hypoxia-NaCl stress was investigated in this study. Exogenous Ca2+ increased GABA content significantly by enhancing glutamate decarboxylase gene expression and its activity. Addition of ethylene ...[more]

PMID: 28911384

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