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Understanding the competing pathways leading to hydropyrene and isoelisabethatriene.

ABSTRACT: Terpene synthases are responsible for the biosynthesis of terpenes, the largest family of natural products. Hydropyrene synthase generates hydropyrene and hydropyrenol as its main products along with two byproducts, isoelisabethatrienes A and B. Fascinatingly, a single active site mutation (M75L) diverts the product distribution towards isoelisabethatrienes A and B. In the current work, we study the competing pathways leading to these products using quantum chemical calculations in the gas phase. We show that there is a great thermodynamic preference for hydropyrene and hydropyrenol formation, and hence most likely in the synthesis of the isoelisabethatriene products kinetic control is at play.

SUBMITTER: Zev S

PROVIDER: S-EPMC9359192 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Understanding the competing pathways leading to hydropyrene and isoelisabethatriene.

Zev Shani S Ringel Marion M Driller Ronja R Loll Bernhard B Brück Thomas T Major Dan T DT

Beilstein journal of organic chemistry 20220804

Terpene synthases are responsible for the biosynthesis of terpenes, the largest family of natural products. Hydropyrene synthase generates hydropyrene and hydropyrenol as its main products along with two byproducts, isoelisabethatrienes A and B. Fascinatingly, a single active site mutation (M75L) diverts the product distribution towards isoelisabethatrienes A and B. In the current work, we study the competing pathways leading to these products using quantum chemical calculations in the gas phase ...[more]

PMID: 35965858

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