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Chloroplast genome of the invasive Pyrus calleryana underscores the high molecular diversity of the species.

ABSTRACT: The complete chloroplast genome of Pyrus calleryana (GenBank OM541581.1) was developed by de novo assembly from whole-genome sequencing data. Reference-guided (P. phaeocarpa) read mapping and assembly were followed by annotation and phylogenetic comparisons. The 159,965 bp P. calleryana chloroplast genome represented 36.56% GC content with a classical quadripartite architecture and two inverted repeats regions (IRs; each 26,392 bp) separating the large single-copy region (LSC; 87,942 bp) and the small single-copy region (SSC; 19.239 bp). In total, 125 unique features were annotated in that genome, including 83 protein coding genes, 38 tRNA coding genes, and 4 rRNA coding genes. Phylogenetic analyses based on the whole chloroplast genome sequences placed the P. calleryana among other Rosaceae plants, specifically among the Asian species of Pyrus.

SUBMITTER: Nowicki M

PROVIDER: S-EPMC9365713 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Chloroplast genome of the invasive Pyrus calleryana underscores the high molecular diversity of the species.

Nowicki Marcin M Huff Matthew L ML Staton Margaret E ME Trigiano Robert N RN

Journal of applied genetics 20220506 3

The complete chloroplast genome of Pyrus calleryana (GenBank OM541581.1) was developed by de novo assembly from whole-genome sequencing data. Reference-guided (P. phaeocarpa) read mapping and assembly were followed by annotation and phylogenetic comparisons. The 159,965 bp P. calleryana chloroplast genome represented 36.56% GC content with a classical quadripartite architecture and two inverted repeats regions (IRs; each 26,392 bp) separating the large single-copy region (LSC; 87,942 bp) and the ...[more]

PMID: 35524105

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Project description:Pyrus calleryana Decne. (Callery pear) is a deciduous tree native to China, Japan, Korea, and Taiwan. It is a popular ornamental tree in the United States (US) with early spring blooms and vibrant fall color. There are at least 26 cultivars of P. calleryana available in the US of which "Bradford" is the most well-known. Open-pollinated P. calleryana escapees are becoming one of the most common invasive tree species in the eastern United States. Developing better management practices for invasive P. calleryana requires detailed knowledge about reproductive biology and genetic diversity of the species, however, little is currently known about genetic variability within those open-pollinated populations. We investigated genetic diversity and population structure of non-cultivated, escaped P. calleryana populations within a ∼177 km radius in the southeastern United States. Because P. calleryana exhibits a range of morphological variation with great evolutionary potential, we hypothesized that a high genetic diversity would be manifested among escaped P. calleryana. Using 15 previously developed microsatellite loci, we genotyped 180 open-pollinated P. calleryana individuals that were collected across six naturally occurring sites in Tennessee, Georgia, and South Carolina, United States. Our results demonstrated the presence of a population structure with high genetic diversity, high gene flow, and high genetic differentiation between individuals across collection sites. Our results revealed that P. calleryana populations had differentiated shortly after the introduction to the US, most likely from specimens imported from Asia, consistent with historical records and our prior findings. The high invasive potential of the species is perhaps best underscored by transformation of P. calleryana specimens introduced from Asia into escape populations at continental scale across the United States. Our data also provided novel insight into potential issues that could be problematic for the future as P. calleryana may pose a potential threat to the economy, ecology, and native biodiversity in invaded areas.

Project description:The pear is an important temperate fruit worldwide that is produced by a group of species in the genus Pyrus. Callery pear (Pyrus calleryana Decne) is characterized by high resistance to multiple diseases, good adaptability, and high ornamental value, and is therefore widely planted in pear orchards for edible fruit production or as stock. Plant pathogens are a major threat to pear yield. Black spot disease, caused by the filamentous fungus Alternaria alternata, is one of the most serious diseases in pear. Elucidation of resistant genes to black spot disease is extremely important for understanding the underlying mechanisms as well as for the development of resistant cultivars. In this study, high-throughput single-strand RNA-sequencing was used to compare the transcriptome profiles of Callery pear leaves before and after A. alternata incubation for 7 days. The analysis yielded 73.3 Gb of clean data that were mapped onto the reference genome of the Chinese pear, and differentially expressed gene(DEG)s were identified with |log2FC| ≥ 1. Functional annotation demonstrated that black spot disease promoted great changes in the overall metabolism, and enrichment analysis of gene ontology terms showed that most of them are closely linked to signalling network and photosynthesis. Specifically, the genes included mainly transcription factors and genes involved in calcium signalling and ethylene and jasmonate pathways. Eight members of the ethylene response factor transcription factor gene family Group IX, including ERF1, ERF7, and ERF105, were up-regulated to 2.03-3.37-fold compared with CK, suggesting their role in the defence response to pathogen infection. Additionally, multiple transcription factors involved in biotic stresses, such as NAC78, NAC2, MYB44, and bHLH28, were up-regulated. Furthermore, we identified 144 long non-coding (lnc)RNAs, providing new insight into the involvement of lncRNAs in the response to black spot disease. Our study provides valuable data on the molecular genetics and functional genomic mechanisms of resistance to black spot disease in Callery pear. A good understanding of the molecular response to this disease will allow the development of durable and environmentally friendly control strategies.

Dataset Information

Chloroplast genome of the invasive Pyrus calleryana underscores the high molecular diversity of the species.

Publications

Chloroplast genome of the invasive Pyrus calleryana underscores the high molecular diversity of the species.

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