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Live Streaming of a Single Cell's Life over a Local pH-Monitoring Nanowire Waveguide.

ABSTRACT: Spatiotemporal pH monitoring of single living cells across rigid cell and organelle membranes has been challenging, despite its significance in understanding cellular heterogeneity. Here, we developed a mechanically robust yet tolerably thin nanowire waveguide that enables in situ monitoring of pH dynamics at desired cellular compartments via direct optical communication. By chemically labeling fluorescein at one end of a poly(vinylbenzyl azide) nanowire, we continuously monitored pH variations of different compartments inside a living cell, successfully observing organelle-exclusive pH homeostasis and stimuli-selective pH regulations. Importantly, it was demonstrated for the first time that, during the mammalian cell cycle, the nucleus displays pH homeostasis in interphase but a tidal pH curve in the mitotic phase, implying the existence of independent pH-regulating activities by the nuclear envelope. The rapid and accurate local pH-reporting capability of our nanowire waveguide would be highly valuable for investigating cellular behaviors under diverse biological situations in living cells.

SUBMITTER: Yong MJ

PROVIDER: S-EPMC9372996 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Live Streaming of a Single Cell's Life over a Local pH-Monitoring Nanowire Waveguide.

Yong Moon-Jung MJ Kang Byunghwa B Yang Un U Oh Seung Soo SS Je Jung Ho JH

Nano letters 20220725 15

Spatiotemporal pH monitoring of single living cells across rigid cell and organelle membranes has been challenging, despite its significance in understanding cellular heterogeneity. Here, we developed a mechanically robust yet tolerably thin nanowire waveguide that enables in situ monitoring of pH dynamics at desired cellular compartments via direct optical communication. By chemically labeling fluorescein at one end of a poly(vinylbenzyl azide) nanowire, we continuously monitored pH variations ...[more]

PMID: 35877544

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Dataset Information

Live Streaming of a Single Cell's Life over a Local pH-Monitoring Nanowire Waveguide.

Publications

Live Streaming of a Single Cell's Life over a Local pH-Monitoring Nanowire Waveguide.

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