Dataset Information

Response of chlorinated hydrocarbon transformation and microbial community structure in an aquifer to joint H₂ and O₂.

ABSTRACT: Hydrogen (H₂) and oxygen (O₂) are critical electron donors and acceptors to promote the anaerobic and aerobic microbial transformation of chlorinated hydrocarbons (CHCs), respectively. Electrochemical technology can effectively supply H₂ and O₂ directly to an aquifer. However, the response of CHC transformation and microbial community structure to joint H₂ and O₂ are still unclear. In this work, microcosms containing different combinations of H₂ and O₂ were constructed with natural sediments and nine mixed CHCs. The joint H₂ and O₂ microcosm (H₂/O₂ microcosm) significantly promoted the biotransformation of trichloroethylene (TCE), trans-dichloroethene (tDCE) and chloroform (CF). Illumina sequencing analyses suggested that a particular microbial community was formed in the H₂/O₂ microcosm. The specific microbial species included Methyloversatilis, Dechloromonas, Sediminibacterium, Pseudomonas, Acinetobacter, Curvibacter, Comamonas and Acidovorax, and the relative abundance of the tceA, phe and soxB genes synchronously increased. These results suggested that some specific microbes are potential CHC converters using H₂ and O₂ as energy sources, and aerobic and anaerobic transformations exist simultaneously in the H₂/O₂ microcosm. It provides a theoretical basis for establishing efficient green remediation technologies for CHC contaminated aquifers.

SUBMITTER: Li C

PROVIDER: S-EPMC9380535 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Response of chlorinated hydrocarbon transformation and microbial community structure in an aquifer to joint H2 and O2.

Li Cui C Chen Rong R Liu Hui H Huang Yao Y Yu Jintao J Ouyang Weiwei W Xue Chen C

RSC advances 20220816 36

Hydrogen (H2) and oxygen (O2) are critical electron donors and acceptors to promote the anaerobic and aerobic microbial transformation of chlorinated hydrocarbons (CHCs), respectively. Electrochemical technology can effectively supply H2 and O2 directly to an aquifer. However, the response of CHC transformation and microbial community structure to joint H2 and O2 are still unclear. In this work, microcosms containing different com ...[more]

PMID: 36090448

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Project description:A culture-independent molecular phylogenetic approach was used to survey constituents of microbial communities associated with an aquifer contaminated with hydrocarbons (mainly jet fuel) and chlorinated solvents undergoing intrinsic bioremediation. Samples were obtained from three redox zones: methanogenic, methanogenic-sulfate reducing, and iron or sulfate reducing. Small-subunit rRNA genes were amplified directly from aquifer material DNA by PCR with universally conserved or Bacteria- or Archaea-specific primers and were cloned. A total of 812 clones were screened by restriction fragment length polymorphisms (RFLP), approximately 50% of which were unique. All RFLP types that occurred more than once in the libraries, as well as many of the unique types, were sequenced. A total of 104 (94 bacterial and 10 archaeal) sequence types were determined. Of the 94 bacterial sequence types, 10 have no phylogenetic association with known taxonomic divisions and are phylogenetically grouped in six novel division level groups (candidate divisions WS1 to WS6); 21 belong to four recently described candidate divisions with no cultivated representatives (OP5, OP8, OP10, and OP11); and 63 are phylogenetically associated with 10 well-recognized divisions. The physiology of two particularly abundant sequence types obtained from the methanogenic zone could be inferred from their phylogenetic association with groups of microorganisms with a consistent phenotype. One of these sequence types is associated with the genus Syntrophus; Syntrophus spp. produce energy from the anaerobic oxidation of organic acids, with the production of acetate and hydrogen. The organism represented by the other sequence type is closely related to Methanosaeta spp., which are known to be capable of energy generation only through aceticlastic methanogenesis. We hypothesize, therefore, that the terminal step of hydrocarbon degradation in the methanogenic zone of the aquifer is aceticlastic methanogenesis and that the microorganisms represented by these two sequence types occur in syntrophic association.

Project description:Knowledge about the relationship between microbial community structure and hydrogeochemistry (e.g., pollution, redox and degradation processes) in landfill leachate-polluted aquifers is required to develop tools for predicting and monitoring natural attenuation. In this study analyses of pollutant and redox chemistry were conducted in parallel with culture-independent profiling of microbial communities present in a well-defined aquifer (Banisveld, The Netherlands). Degradation of organic contaminants occurred under iron-reducing conditions in the plume of pollution, while upstream of the landfill and above the plume denitrification was the dominant redox process. Beneath the plume iron reduction occurred. Numerical comparison of 16S ribosomal DNA (rDNA)-based denaturing gradient gel electrophoresis (DGGE) profiles of Bacteria and Archaea in 29 groundwater samples revealed a clear difference between the microbial community structures inside and outside the contaminant plume. A similar relationship was not evident in sediment samples. DGGE data were supported by sequencing cloned 16S rDNA. Upstream of the landfill members of the beta subclass of the class Proteobacteria (beta-proteobacteria) dominated. This group was not encountered beneath the landfill, where gram-positive bacteria dominated. Further downstream the contribution of gram-positive bacteria to the clone library decreased, while the contribution of delta-proteobacteria strongly increased and beta-proteobacteria reappeared. The beta-proteobacteria (Acidovorax, Rhodoferax) differed considerably from those found upstream (Gallionella, Azoarcus). Direct comparisons of cloned 16S rDNA with bands in DGGE profiles revealed that the data from each analysis were comparable. A relationship was observed between the dominant redox processes and the bacteria identified. In the iron-reducing plume members of the family Geobacteraceae made a strong contribution to the microbial communities. Because the only known aromatic hydrocarbon-degrading, iron-reducing bacteria are Geobacter spp., their occurrence in landfill leachate-contaminated aquifers deserves more detailed consideration.

Dataset Information

Response of chlorinated hydrocarbon transformation and microbial community structure in an aquifer to joint H₂ and O₂.

Publications

Response of chlorinated hydrocarbon transformation and microbial community structure in an aquifer to joint H<sub>2</sub> and O<sub>2</sub>.

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