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Cloning of the glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa.


ABSTRACT: The glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa was identified. A plasmid containing a 2.3-kb insert complemented the temperature-sensitive gltX mutation of Escherichia coli JP1449, and GltX activity was demonstrated. The inferred amino acid sequence of this gene showed 50.6% identity with GltX from Rhizobium meliloti.

SUBMITTER: Franklund CV 

PROVIDER: S-EPMC93828 | biostudies-literature | 1999 Jun

REPOSITORIES: biostudies-literature

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Cloning of the glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa.

Franklund C V CV   Goldberg J B JB  

Journal of bacteriology 19990601 11


The glutamyl-tRNA synthetase (gltX) gene from Pseudomonas aeruginosa was identified. A plasmid containing a 2.3-kb insert complemented the temperature-sensitive gltX mutation of Escherichia coli JP1449, and GltX activity was demonstrated. The inferred amino acid sequence of this gene showed 50.6% identity with GltX from Rhizobium meliloti. ...[more]

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