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Identification of two new proteins in spermidine nucleoids isolated from Escherichia coli.

ABSTRACT: The Escherichia coli nucleoid contains DNA in a condensed but functional form. Analysis of proteins released from isolated spermidine nucleoids after treatment with DNase I reveals significant amounts of two proteins not previously detected in wild-type E. coli. Partial amino-terminal sequencing has identified them as the products of rdgC and yejK. These proteins are strongly conserved in gram-negative bacteria, suggesting that they have important cellular roles.

SUBMITTER: Murphy LD

PROVIDER: S-EPMC93866 | biostudies-literature | 1999 Jun

REPOSITORIES: biostudies-literature

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Identification of two new proteins in spermidine nucleoids isolated from Escherichia coli.

Murphy L D LD Rosner J L JL Zimmerman S B SB Esposito D D

Journal of bacteriology 19990601 12

The Escherichia coli nucleoid contains DNA in a condensed but functional form. Analysis of proteins released from isolated spermidine nucleoids after treatment with DNase I reveals significant amounts of two proteins not previously detected in wild-type E. coli. Partial amino-terminal sequencing has identified them as the products of rdgC and yejK. These proteins are strongly conserved in gram-negative bacteria, suggesting that they have important cellular roles. ...[more]

PMID: 10368163

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