Cloning and characterization of the gene encoding Pasteurella haemolytica FnrP, a regulator of the Escherichia coli silent hemolysin sheA.
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ABSTRACT:
SUBMITTER: Uhlich GA
PROVIDER: S-EPMC93867 | biostudies-literature | 1999 Jun
REPOSITORIES: biostudies-literature
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Cloning and characterization of the gene encoding Pasteurella haemolytica FnrP, a regulator of the Escherichia coli silent hemolysin sheA.
Uhlich G A GA McNamara P J PJ Iandolo J J JJ Mosier D A DA
Journal of bacteriology 19990601 12
A Pasteurella haemolytica A1 gene was identified from a recombinant library clone that expressed hemolysis in host Escherichia coli cells. The gene, designated fnrP, had sequence identity to E. coli fnr, a global transcriptional regulator of genes required for conversion to anaerobic growth. FnrP complemented anaerobic deficiencies of a fnr-null mutant strain of E. coli and increased expression of the Fnr-dependent, anaerobic terminal reductase gene, frdA. FnrP was purified, identified by immuno ...[more]
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