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Establishment of Neurotoxicity Assessment Using Microelectrode Array (MEA) with hiPSC-Derived Neurons and Evaluation of New Psychoactive Substances (NPS).


ABSTRACT:

Background and objectives

Currently, safety pharmacological tests for the central nervous system depend on animal behavioral analysis. However, due to the subjectivity of behavioral analysis and differences between species, there is a limit to appropriate nervous system toxicity assessment, therefore a new neurotoxicity assessment that can simulate the human central nervous system is required.

Methods and results

In our study, we developed an in vitro neurotoxicity assessment focusing on neuronal function. To minimize the differences between species and fast screening, hiPSC-derived neurons and a microelectrode array (MEA) that could simultaneously measure the action potentials of the neuronal networks were used. After analyzing the molecular and electrophysiological characters of our neuronal network, we conducted a neurotoxicity assessment on neurotransmitters, neurotoxicants, illicit drugs, and new psychoactive substances (NPS). We found that most substances used in our experiments responded more sensitively to our MEA-based neurotoxicity assessment than to the conventional neurotoxicity assessment. Also, this is the first paper that evaluates various illicit drugs and NPS using MEA-based neurotoxicity assessment using hiPSC-derived neurons.

Conclusions

Our study expanded the scope of application of neurotoxicity assessment using hiPSC-derived neurons to NPS, and accumulated evaluation data of various toxic substances for hiPSC-derived neurons.

SUBMITTER: Kang KR 

PROVIDER: S-EPMC9396014 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Publications

Establishment of Neurotoxicity Assessment Using Microelectrode Array (MEA) with hiPSC-Derived Neurons and Evaluation of New Psychoactive Substances (NPS).

Kang Kyu-Ree KR   Kim C-Yoon CY   Kim Jin J   Ryu Bokyeong B   Lee Seul-Gi SG   Baek Jieun J   Kim Ye-Ji YJ   Lee Jin-Moo JM   Lee Yootmo Y   Choi Sun-Ok SO   Woo Dong Ho DH   Park Il Hwan IH   Chung Hyung Min HM  

International journal of stem cells 20220630 3


<h4>Background and objectives</h4>Currently, safety pharmacological tests for the central nervous system depend on animal behavioral analysis. However, due to the subjectivity of behavioral analysis and differences between species, there is a limit to appropriate nervous system toxicity assessment, therefore a new neurotoxicity assessment that can simulate the human central nervous system is required.<h4>Methods and results</h4>In our study, we developed an in vitro neurotoxicity assessment focu  ...[more]

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