Ontology highlight
ABSTRACT: Background and objectives
Currently, safety pharmacological tests for the central nervous system depend on animal behavioral analysis. However, due to the subjectivity of behavioral analysis and differences between species, there is a limit to appropriate nervous system toxicity assessment, therefore a new neurotoxicity assessment that can simulate the human central nervous system is required.Methods and results
In our study, we developed an in vitro neurotoxicity assessment focusing on neuronal function. To minimize the differences between species and fast screening, hiPSC-derived neurons and a microelectrode array (MEA) that could simultaneously measure the action potentials of the neuronal networks were used. After analyzing the molecular and electrophysiological characters of our neuronal network, we conducted a neurotoxicity assessment on neurotransmitters, neurotoxicants, illicit drugs, and new psychoactive substances (NPS). We found that most substances used in our experiments responded more sensitively to our MEA-based neurotoxicity assessment than to the conventional neurotoxicity assessment. Also, this is the first paper that evaluates various illicit drugs and NPS using MEA-based neurotoxicity assessment using hiPSC-derived neurons.Conclusions
Our study expanded the scope of application of neurotoxicity assessment using hiPSC-derived neurons to NPS, and accumulated evaluation data of various toxic substances for hiPSC-derived neurons.
SUBMITTER: Kang KR
PROVIDER: S-EPMC9396014 | biostudies-literature | 2022 Aug
REPOSITORIES: biostudies-literature
Kang Kyu-Ree KR Kim C-Yoon CY Kim Jin J Ryu Bokyeong B Lee Seul-Gi SG Baek Jieun J Kim Ye-Ji YJ Lee Jin-Moo JM Lee Yootmo Y Choi Sun-Ok SO Woo Dong Ho DH Park Il Hwan IH Chung Hyung Min HM
International journal of stem cells 20220630 3
<h4>Background and objectives</h4>Currently, safety pharmacological tests for the central nervous system depend on animal behavioral analysis. However, due to the subjectivity of behavioral analysis and differences between species, there is a limit to appropriate nervous system toxicity assessment, therefore a new neurotoxicity assessment that can simulate the human central nervous system is required.<h4>Methods and results</h4>In our study, we developed an in vitro neurotoxicity assessment focu ...[more]