Cloning and characterization of a sulfonate/alpha-ketoglutarate dioxygenase from Saccharomyces cerevisiae.
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ABSTRACT:
SUBMITTER: Hogan DA
PROVIDER: S-EPMC94115 | biostudies-literature | 1999 Sep
REPOSITORIES: biostudies-literature
Publications
Cloning and characterization of a sulfonate/alpha-ketoglutarate dioxygenase from Saccharomyces cerevisiae.
Hogan D A DA Auchtung T A TA Hausinger R P RP
Journal of bacteriology 19990901 18
The Saccharomyces cerevisiae open reading frame YLL057c is predicted to encode a gene product with 31.5% amino acid sequence identity to Escherichia coli taurine/alpha-ketoglutarate dioxygenase and 27% identity to Ralstonia eutropha TfdA, a herbicide-degrading enzyme. Purified recombinant yeast protein is shown to be an Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase. Although taurine is a poor substrate, a variety of other sulfonates are utilized, with the best natural substrates bei ...[more]
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