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Purification of quantum dot-based bioprobes with a salting out strategy.


ABSTRACT: A salting out strategy is reported for purification of IgG-conjugated QD (IgG-QD) bioprobes. Adding NaCl can precipitate free IgG selectively, while the IgG-QD maintains good colloidal stability. The dynamic light scattering technique reveals that this is due to the relatively positive zeta potential of free IgG than that of the IgG-QD.

SUBMITTER: Chen ZL 

PROVIDER: S-EPMC9417310 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Purification of quantum dot-based bioprobes with a salting out strategy.

Chen Zhi-Liang ZL   Xu Jia-Quan JQ  

Nanoscale advances 20220104 2


A salting out strategy is reported for purification of IgG-conjugated QD (IgG-QD) bioprobes. Adding NaCl can precipitate free IgG selectively, while the IgG-QD maintains good colloidal stability. The dynamic light scattering technique reveals that this is due to the relatively positive zeta potential of free IgG than that of the IgG-QD. ...[more]

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