Ontology highlight
ABSTRACT:
SUBMITTER: Mortimer MW
PROVIDER: S-EPMC94220 | biostudies-literature | 1999 Dec
REPOSITORIES: biostudies-literature
Mortimer M W MW McDermott T R TR York G M GM Walker G C GC Kahn M L ML
Journal of bacteriology 19991201 24
The gltA gene, encoding Sinorhizobium meliloti 104A14 citrate synthase, was isolated by complementing an Escherichia coli gltA mutant. The S. meliloti gltA gene was mutated by inserting a kanamycin resistance gene and then using homologous recombination to replace the wild-type gltA with the gltA::kan allele. The resulting strain, CSDX1, was a glutamate auxotroph, and enzyme assays confirmed the absence of a requirement for glutamate. CSDX1 did not grow on succinate, malate, aspartate, pyruvate, ...[more]