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Pharmacological investigation of brucine anti-ulcer potential.

ABSTRACT: Gastric ulcer is one of the most common chronic gastrointestinal diseases characterized by a significant defect in the mucosal barrier. The current study has been conducted to evaluate the brucine anti-ulcer effect. Brucine has binding energy values ranging from -2.99 to -8.11 kcal/mol against chosen targets, according to in silico research. Brucine exhibits an inhibitory effect against Helicobacter pylori. In vivo findings revealed that brucine (3 mg/kg) showed effective results in healing ethanol-induced ulcer lesions of the gastric region in rats. Brucine showed an inhibitory effect against H⁺/K⁺-ATPase. Levels of glutathione, glutathione-s-transferase, and catalase were enhanced in the gastric rat tissue with the use of brucine, while a significant decrease in lipid peroxide levels was seen. Histopathological evaluation showed improvement in cellular architecture and a decrease in inflammatory indicators like cyclooxygenase, tumor necrosis factor, and nuclear factor kappa B expression, validated through immunohistochemistry, enzyme-linked immunosorbent assay, and Western blot techniques. In the reverse transcription-polymerase chain reaction, brucine decreased H⁺/K⁺-ATPase mRNA levels. This study reveals that brucine possesses stable binding affinities against selected targets. Brucine exhibits an anti-ulcer effect, mediated via anti-H. pylori, H⁺/K⁺-ATPase inhibition, and antioxidant and anti-inflammatory pathways.

SUBMITTER: Noman M

PROVIDER: S-EPMC9429807 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Pharmacological investigation of brucine anti-ulcer potential.

Noman Muhammad M Qazi Neelum Gul NG Rehman Najeeb Ur NU Khan Arif-Ullah AU

Frontiers in pharmacology 20220817

Gastric ulcer is one of the most common chronic gastrointestinal diseases characterized by a significant defect in the mucosal barrier. The current study has been conducted to evaluate the brucine anti-ulcer effect. Brucine has binding energy values ranging from -2.99 to -8.11 kcal/mol against chosen targets, according to in silico research. Brucine exhibits an inhibitory effect against Helicobacter pylori. In vivo findings revealed that brucine (3 mg/kg) showed effective re ...[more]

PMID: 36059979

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Pharmacological investigation of brucine anti-ulcer potential.

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Pharmacological investigation of brucine anti-ulcer potential.

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