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Correction of the DNA sequence of the regB gene of Rhodobacter capsulatus with implications for the membrane topology of the sensor kinase regB.

ABSTRACT: We corrected the previously published sequence for the regB gene, which encodes a histidine sensor kinase in Rhodobacter capsulatus. The deduced RegB amino acid sequence has an additional putative transmembrane domain at the N terminus. Analysis of RegB-PhoA and RegB-LacZ fusion proteins supports a topology model for RegB with six membrane-spanning domains.

SUBMITTER: Chen W

PROVIDER: S-EPMC94348 | biostudies-literature | 2000 Feb

REPOSITORIES: biostudies-literature

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Correction of the DNA sequence of the regB gene of Rhodobacter capsulatus with implications for the membrane topology of the sensor kinase regB.

Chen W W Jäger A A Klug G G

Journal of bacteriology 20000201 3

We corrected the previously published sequence for the regB gene, which encodes a histidine sensor kinase in Rhodobacter capsulatus. The deduced RegB amino acid sequence has an additional putative transmembrane domain at the N terminus. Analysis of RegB-PhoA and RegB-LacZ fusion proteins supports a topology model for RegB with six membrane-spanning domains. ...[more]

PMID: 10633119

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