Ontology highlight
ABSTRACT:
SUBMITTER: Hansen SR
PROVIDER: S-EPMC9436412 | biostudies-literature | 2022 Aug
REPOSITORIES: biostudies-literature
Hansen Sarah R SR White David S DS Scalf Mark M Corrêa Ivan R IR Smith Lloyd M LM Hoskins Aaron A AA
eLife 20220812
In eukaryotes, splice sites define the introns of pre-mRNAs and must be recognized and excised with nucleotide precision by the spliceosome to make the correct mRNA product. In one of the earliest steps of spliceosome assembly, the U1 small nuclear ribonucleoprotein (snRNP) recognizes the 5' splice site (5' SS) through a combination of base pairing, protein-RNA contacts, and interactions with other splicing factors. Previous studies investigating the mechanisms of 5' SS recognition have largely ...[more]