MiRSCAPE - inferring miRNA expression from scRNA-seq data.
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ABSTRACT:
SUBMITTER: Olgun G
PROVIDER: S-EPMC9437856 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
Publications
miRSCAPE - inferring miRNA expression from scRNA-seq data.
Olgun Gulden G Gopalan Vishaka V Hannenhalli Sridhar S
iScience 20220817 9
Our understanding of miRNA activity at cellular resolution is thwarted by the inability of standard scRNA-seq protocols to capture miRNAs. We introduce a novel tool, miRSCAPE, to infer miRNA expression in a sample from its RNA-seq profile. We establish miRSCAPE's accuracy in 10 tumor and normal cohorts demonstrating its superiority over alternatives. miRSCAPE accurately infers cell type-specific miRNA activities (predicted versus observed fold-difference correlation ∼0.81) in two independent scR ...[more]
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