Priming enzymes from the pikromycin synthase reveal how assembly-line ketosynthases catalyze carbon-carbon chemistry.

ABSTRACT: The first domain of modular polyketide synthases (PKSs) is most commonly a ketosynthase (KS)-like enzyme, KS^Q, that primes polyketide synthesis. Unlike downstream KSs that fuse α-carboxyacyl groups to growing polyketide chains, it performs an extension-decoupled decarboxylation of these groups to generate primer units. When Pik127, a model triketide synthase constructed from modules of the pikromycin synthase, was studied by cryoelectron microscopy (cryo-EM), the dimeric didomain comprised of KS^Q and the neighboring methylmalonyl-selective acyltransferase (AT) dominated the class averages and yielded structures at 2.5- and 2.8-Å resolution, respectively. Comparisons with ketosynthases complexed with their substrates revealed the conformation of the (2S)-methylmalonyl-S-phosphopantetheinyl portion of KS^Q and KS substrates prior to decarboxylation. Point mutants of Pik127 probed the roles of residues in the KS^Q active site, while an AT-swapped version of Pik127 demonstrated that KS^Q can also decarboxylate malonyl groups. Mechanisms for how KS^Q and KS domains catalyze carbon-carbon chemistry are proposed.

SUBMITTER: Dickinson MS 

PROVIDER: S-EPMC9444953 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature