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Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids.

ABSTRACT: Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease in vitro. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells. For complete details on the use and execution of this protocol, please refer to Cujba et al. (2022).

SUBMITTER: Pedraza-Arevalo S

PROVIDER: S-EPMC9449863 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Differentiation of beta-like cells from human induced pluripotent stem cell-derived pancreatic progenitor organoids.

Pedraza-Arevalo Sergio S Cujba Ana-Maria AM Alvarez-Fallas Mario Enrique ME Sancho Rocio R

STAR protocols 20220830 3

Human induced pluripotent stem cells (hiPSCs) and organoids are important for modeling human development and disease <i>in vitro</i>. In this study, we describe a protocol to differentiate hiPSC toward pancreatic progenitor (PP) organoids and beta-like cells. We detail the expansion and seeding of hiPSC, PP differentiation, organoid expansion, and the differentiation of PP into beta cells. Upon differentiation, organoids contained beta, delta, and alpha cells. For complete details on the use and ...[more]

PMID: 36092820

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