Ontology highlight
ABSTRACT:
SUBMITTER: Brinkman FS
PROVIDER: S-EPMC94676 | biostudies-literature | 2000 Sep
REPOSITORIES: biostudies-literature
Brinkman F S FS Bains M M Hancock R E RE
Journal of bacteriology 20000901 18
Pseudomonas aeruginosa OprF forms 0.36-nS channels and, rarely, 2- to 5-nS channels in lipid bilayer membranes. We show that a protein comprising only the N-terminal 162-amino-acid domain of OprF formed the smaller, but not the larger, channels in lipid bilayers. Circular dichroism spectroscopy indicated that this protein folds into a beta-sheet-rich structure, and three-dimensional comparative modeling revealed that it shares significant structural similarity with the amino terminus of the orth ...[more]