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Electrochemical Capillary Driven Immunoassay for Detection of SARS-CoV-2.

ABSTRACT: The COVID-19 pandemic focused attention on a pressing need for fast, accurate, and low-cost diagnostic tests. This work presents an electrochemical capillary driven immunoassay (eCaDI) developed to detect SARS-CoV-2 nucleocapsid (N) protein. The low-cost flow device is made of polyethylene terephthalate (PET) and adhesive films. Upon addition of a sample, reagents and washes are sequentially delivered to an integrated screen-printed carbon electrode for detection, thus automating a full sandwich immunoassay with a single end-user step. The modified electrodes are sensitive and selective for SARS-CoV-2 N protein and stable for over 7 weeks. The eCaDI was tested with influenza A and Sindbis virus and proved to be selective. The eCaDI was also successfully applied to detect nine different SARS-CoV-2 variants, including Omicron.

SUBMITTER: Clark KM

PROVIDER: S-EPMC9469961 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Electrochemical Capillary Driven Immunoassay for Detection of SARS-CoV-2.

Clark Kaylee M KM Schenkel Melissa S MS Pittman Trey W TW Samper Isabelle C IC Anderson Loran B R LBR Khamcharoen Wisarut W Elmegerhi Suad S Perera Rushika R Siangproh Weena W Kennan Alan J AJ Geiss Brian J BJ Dandy David S DS Henry Charles S CS

ACS measurement science au 20220830 6

The COVID-19 pandemic focused attention on a pressing need for fast, accurate, and low-cost diagnostic tests. This work presents an electrochemical capillary driven immunoassay (eCaDI) developed to detect SARS-CoV-2 nucleocapsid (N) protein. The low-cost flow device is made of polyethylene terephthalate (PET) and adhesive films. Upon addition of a sample, reagents and washes are sequentially delivered to an integrated screen-printed carbon electrode for detection, thus automating a full sandwich ...[more]

PMID: 36570470

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