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Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion.

ABSTRACT: We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.

SUBMITTER: Jumeaux C

PROVIDER: S-EPMC9473185 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Detection of microRNA biomarkers <i>via</i> inhibition of DNA-mediated liposome fusion.

Jumeaux Coline C Kim Eunjung E Howes Philip D PD Kim Hyemin H Chandrawati Rona R Stevens Molly M MM

Nanoscale advances 20181121 2

We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target. ...[more]

PMID: 36132259

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