Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli.
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ABSTRACT:
SUBMITTER: Miller S
PROVIDER: S-EPMC94807 | biostudies-literature | 2000 Nov
REPOSITORIES: biostudies-literature
Publications
Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli.
Miller S S Ness L S LS Wood C M CM Fox B C BC Booth I R IR
Journal of bacteriology 20001101 22
A new subunit, YabF, for the KefC K(+) efflux system in Escherichia coli has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations. ...[more]
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