Ontology highlight
ABSTRACT:
SUBMITTER: Minakhin L
PROVIDER: S-EPMC94851 | biostudies-literature | 2001 Jan
REPOSITORIES: biostudies-literature
Minakhin L L Nechaev S S Campbell E A EA Severinov K K
Journal of bacteriology 20010101 1
The three-dimensional structure of DNA-dependent RNA polymerase (RNAP) from thermophilic Thermus aquaticus has recently been determined at 3.3 A resolution. Currently, very little is known about T. aquaticus transcription and no genetic system to study T. aquaticus RNAP genes is available. To overcome these limitations, we cloned and overexpressed T. aquaticus RNAP genes in Escherichia coli. Overproduced T. aquaticus RNAP subunits assembled into functional RNAP in vitro and in vivo when coexpres ...[more]