The KlGpa1 gene encodes a G-protein alpha subunit that is a positive control element in the mating pathway of the budding yeast Kluyveromyces lactis.

ABSTRACT: The cloning of the gene encoding the KlGpa1p subunit was achieved by standard PCR techniques and by screening a Kluyveromyces lactis genomic library using the PCR product as a probe. The full-length open reading frame spans 1,344 nucleotides including the stop codon. The deduced primary structure of the protein (447 amino acid residues) strongly resembles that of Gpa1p, the G-protein alpha subunit from Saccharomyces cerevisiae involved in the mating pheromone response pathway. Nevertheless, unlike disruption of Gpa1 from S. cerevisiae, disruption of KlGpa1 rendered viable cells with a reduced capacity to mate. Expression of a plasmidic KlGpa1 copy in a DeltaKlgpa1 mutant restores full mating competence; hence we conclude that KlGpa1p plays a positive role in the mating pathway. Overexpress

SUBMITTER: Savinon-Tejeda AL 

PROVIDER: S-EPMC94870 | biostudies-literature | 2001 Jan

REPOSITORIES: biostudies-literature