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Prediction of Symptomatic Severe Acute Respiratory Syndrome Coronavirus 2 Infection by Quantitative Digital Polymerase Chain Reaction Normalized to International Units


ABSTRACT: Abstract Although numerous studies have evaluated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection using cycle threshold (Ct) values as a surrogate of viral ribonucleic acid (RNA) load, few studies have used standardized, quantitative methods. We validated a quantitative SARS-CoV-2 digital polymerase chain reaction assay normalized to World Health Organization International Units and correlated viral RNA load with symptoms and disease severity. Studies have evaluated SARS-CoV-2 infection using Cycle Thresholds (Ct) values as a surrogate of viral RNA load. We validated a quantitative SARS-CoV-2 digital PCR assay normalized to WHO International Units and correlated viral RNA load with symptoms and disease severity.

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PROVIDER: S-EPMC9494499 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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