Dataset Information

Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model.

ABSTRACT: The current pandemic caused by severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has encouraged the evaluation of novel instruments for disinfection and lowering infectious pressure. Ultraviolet subtype C (UVC) excimer lamps with 222 nm wavelength have been tested on airborne pathogens on surfaces and the exposure to this wavelength has been considered safer than conventional UVC. To test the efficacy of UVC excimer lamps on coronaviruses, an animal model mimicking the infection dynamics was implemented. An attenuated vaccine based on infectious bronchitis virus (IBV) was nebulized and irradiated by 222 nm UVC rays before the exposure of a group of day-old chicks to evaluate the virus inactivation. A control group of chicks was exposed to the nebulized vaccine produced in the same conditions but not irradiated by the lamps. The animals of both groups were sampled daily and individually by choanal cleft swabs and tested usign a strain specific real time RT-PCR to evaluate the vaccine replication. Only the birds in the control group were positive, showing an active replication of the vaccine, revealing the efficacy of the lamps in inactivating the vaccine below the infectious dose in the other group.

SUBMITTER: Tucciarone CM

PROVIDER: S-EPMC9503014 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model.

Tucciarone Claudia Maria CM Cecchinato Mattia M Vianello Lucrezia L Simi Gabriele G Borsato Enrico E Silvestrin Luca L Giorato Michele M Salata Cristiano C Morandin Mauro M Greggio Elisa E Drigo Michele M

Viruses 20220914 9

The current pandemic caused by severe acute respiratory syndrome-related coronavirus-2 (SARS-CoV-2) has encouraged the evaluation of novel instruments for disinfection and lowering infectious pressure. Ultraviolet subtype C (UVC) excimer lamps with 222 nm wavelength have been tested on airborne pathogens on surfaces and the exposure to this wavelength has been considered safer than conventional UVC. To test the efficacy of UVC excimer lamps on coronaviruses, an animal model mimicking the infecti ...[more]

PMID: 36146846

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Project description:Effective disinfection of contaminated surfaces is essential for preventing the transmission of pathogens. In this study, we investigated the UV irradiance and wavelength distribution of a 222-nm ultraviolet C (UVC) excimer lamp and its disinfection efficacy against microorganisms in laboratory conditions. By using a carrier quantitative germicidal test with stainless steel sheets as carriers, we examined the disinfection effect of the 222-nm UVC lamp on three standard strains-Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. We tested the disinfection efficacy under different conditions by adjusting irradiation time, as well as the state and temperature of the stainless steel carriers. Our results indicated that a bacterial suspension in PBS and not-dried stainless steel carriers yielded better disinfection than in TSB and dried carriers. Additionally, carrier temperature had no significant impact on disinfection efficacy. When utilizing a bacterial suspension in PBS and non-dried carriers at a temperature of 20 °C, the three bacteria were eliminated by 222-nm UVC excimer lamp irradiation in just 15 s. In contrast, when using a bacterial suspension in TSB and dried carriers at temperatures of 20 °C, 4 °C, or - 20 °C, the three bacteria were eradicated by 222-nm UVC excimer lamp irradiation in 60 s. Comparatively, the LPM lamp required more than 10 min to achieve the same disinfection effect. Our data demonstrate that the 222-nm UVC excimer lamp has higher irradiance and a more potent microbial disinfection effect than the LPM lamp, requiring significantly less irradiation time to achieve the same disinfection effect under identical conditions. Furthermore, the 222-nm UVC excimer lamp exhibited a substantial disinfection effect on bacterial propagules at low temperatures. Our findings support the optimization of "tunnel-type" cold-chain goods disinfection devices, providing an alternative, highly efficient, and practical tool to combat the spread of SARS-CoV-2 through cold-chain systems.

Project description:In recent years, there has been a gradual increase in the prevalence of drug-resistant bacteria, primarily attributed to the widespread use of antibiotics. This has resulted in heightened mortality rates, morbidity, and exorbitant healthcare costs associated with antibiotic-resistant bacterial infections. In order to mitigate the spread of antibiotic-resistant bacteria, environmental disinfection plays a crucial role. Ultraviolet radiation C (UVC) light disinfection has emerged as a potent technique to limit the transmission of nosocomial pathogens and prevent healthcare-associated infections. Different types of high-touch surfaces were used. A serial disinfected experiment with different 222 nm UVC dosages was conducted on clinically isolated antibiotic-resistant bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus species (VRE), carbapenem-resistant Escherichia coli (CREC), carbapenem-resistant Klebsiella pneumonia (CRKP), carbapenem-resistant Acinetobacter baumannii (CRAB), and carbapenem-resistant Pseudomonas aeruginosa (CRPA) on different material surfaces. The bactericidal efficacy was evaluated by The Clinical & Laboratory Standards Institute (CLSI) guidelines. 222 nm UVC irradiation had a potent bactericidal efficacy on clinical antibiotic-resistant bacteria on different high-touch surfaces that are commonly found in the environment and healthcare facilities. 222 nm UVC irradiation time was tested from 10 s to 1 h. Different surfaces affect the efficiency of 222 nm UVC. The more adsorptive a material is, the higher the dosage of 222 nm UVC irradiation energy is required for effective disinfection. The use of 222 nm UVC lamps for disinfection on different materials has been shown to be a useful method. However, it is crucial to pay attention to the energy required for effective sterilization.ImportanceThis study is crucial, providing compelling evidence on Far-ultraviolet radiation C (Far-UVC) light's efficacy against clinically significant antibiotic-resistant bacteria-a pressing issue in microbiology and infection control. Our research employs antibiotic-resistant strains from clinically isolated bacteria, emphasizing real-world relevance. Simultaneously, we assess Far-UVC light (222 nm) across diverse material surfaces commonly found in clinical settings. This dual approach ensures practical applicability and broad relevance. Our comprehensive setup and rigorous methodologies unequivocally demonstrate Far-UVC light's potency in combating antibiotic-resistant bacteria. Since 222 nm far-UVC has a disinfection capability and is harmless to mammalian cells, this dual effectiveness positions Far-UVC as a secure tool for infection control, with potential applications in healthcare settings, mitigating antibiotic-resistant bacteria spread, and reducing healthcare-associated infections.

Dataset Information

Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model.

Publications

Evaluation of UVC Excimer Lamp (222 nm) Efficacy for Coronavirus Inactivation in an Animal Model.

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