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QPCRtools: An R package for qPCR data processing and visualization.

ABSTRACT: In biological research, qPCR is a technique that is frequently used to measure gene expression levels. The calculation of gene amplification efficiency is a critical step in the processing of qPCR data since it helps to decide which method to employ to compute gene expression levels. Here, we introduce the R package qPCRtools, which enables users to analyze the efficiency of gene amplification. Additionally, this software can determine gene expression levels using one of three approaches: the conventional curve-based method, the 2^-ΔΔCt method, and the SATQPCR method. The qPCRtools package produces a table with the statistical data of each method as well as a figure with a box or bar plot illustrating the results. The R package qPCRtools is freely available at CRAN (https://CRAN.R-project.org/package=qPCRtools) or GitHub (https://github.com/lixiang117423/qPCRtools/tree/main/CRAN/qPCRtools).

SUBMITTER: Li X

PROVIDER: S-EPMC9513427 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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qPCRtools: An R package for qPCR data processing and visualization.

Li Xiang X Wang Yingmin Y Li Jingyu J Mei Xinyue X Liu Yixiang Y Huang Huichuan H

Frontiers in genetics 20220913

In biological research, qPCR is a technique that is frequently used to measure gene expression levels. The calculation of gene amplification efficiency is a critical step in the processing of qPCR data since it helps to decide which method to employ to compute gene expression levels. Here, we introduce the R package qPCRtools, which enables users to analyze the efficiency of gene amplification. Additionally, this software can determine gene expression levels using one of three approaches: the co ...[more]

PMID: 36176301

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