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GlnD and mviN are genes of an essential operon in Sinorhizobium meliloti.


ABSTRACT: To evaluate the role of uridylyl-transferase, the Sinorhizobium meliloti glnD gene was isolated by heterologous complementation in Azotobacter vinelandii. The glnD gene is cotranscribed with a gene homologous to Salmonella mviN. glnD1::Omega or mviN1::Omega mutants could not be isolated by a powerful sucrose counterselection procedure unless a complementing cosmid was provided, indicating that glnD and mviN are members of an indispensable operon in S. meliloti.

SUBMITTER: Rudnick PA 

PROVIDER: S-EPMC95188 | biostudies-literature | 2001 Apr

REPOSITORIES: biostudies-literature

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glnD and mviN are genes of an essential operon in Sinorhizobium meliloti.

Rudnick P A PA   Arcondéguy T T   Kennedy C K CK   Kahn D D  

Journal of bacteriology 20010401 8


To evaluate the role of uridylyl-transferase, the Sinorhizobium meliloti glnD gene was isolated by heterologous complementation in Azotobacter vinelandii. The glnD gene is cotranscribed with a gene homologous to Salmonella mviN. glnD1::Omega or mviN1::Omega mutants could not be isolated by a powerful sucrose counterselection procedure unless a complementing cosmid was provided, indicating that glnD and mviN are members of an indispensable operon in S. meliloti. ...[more]

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