Dataset Information

Classification of Southeast Asian mints (Mentha spp.) based on simple sequence repeat markers.

ABSTRACT: Mentha is a complex genus encompassing many species as a consequence of their interspecific hybridization and polyploidy. Southeast Asian mints have been poorly distinguished though they are widely used for culinary and medical purposes. In this study, we have analyzed Southeast Asian mints and known varieties as well as a related Lamiaceae species (Nepeta sp.) using simple sequence repeat (SSR) markers and leaf morphology. Two types of mints were clearly distinguished based on their venation pattern and leaf shape index. We developed 12 SSR markers that allowed good amplification in the Mentha and another Lamiaceae species. In the SSR-based phylogram, the Mentha lines could be delimited into groups I-VI. The Southeast Asian mints divided into groups I and II, and the phylogram separated most of the available species, with groups I and II containing the known species M. × cordifolia and M. arvensis, respectively. The separation of the two groups was supported by a population structure analysis. The SSR markers developed in this study enabled the simultaneous classification of mints and will help improve our understanding of the genetic composition of known mint varieties and as yet unclassified Southeast Asian mints.

SUBMITTER: Fukui Y

PROVIDER: S-EPMC9522532 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Classification of Southeast Asian mints (Mentha spp.) based on simple sequence repeat markers.

Fukui Yuri Y Saito Moeko M Nakamura Natsuno N Mizuno Taichi T Sato Shuichi S Tsukuda Mayu M Nakaoka Saori S Tsuboi Keita K Sasaki Azusa A Kuramochi Kouji K Boonyaritthongchai Panida P Kaewmanee Nichapat N Thirapanmethee Krit K Chomnawang Mullika Traidej MT Bongcheewin Bhanubong B Nguyen Thuy Linh TL Nguyen Huong Lan Thi HLT Le Huong Thi HT Okamoto Shigehisa S Nakamura Takako T Nakamura Yasushi Y Kubo Nakao N

Breeding science 20220309 2

Mentha is a complex genus encompassing many species as a consequence of their interspecific hybridization and polyploidy. Southeast Asian mints have been poorly distinguished though they are widely used for culinary and medical purposes. In this study, we have analyzed Southeast Asian mints and known varieties as well as a related Lamiaceae species (Nepeta sp.) using simple sequence repeat (SSR) markers and leaf morphology. Two types of mints were clearly distinguished based on the ...[more]

PMID: 36275937

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Project description:Claviceps purpurea is a pathogen that infects most members of Pooideae, a subfamily of Poaceae, and causes ergot, a floral disease in which the ovary is replaced with a sclerotium. When the ergot body is accidently consumed by either man or animal in high enough quantities, there is extreme pain, limb loss and sometimes death.This study was initiated to develop simple sequence repeat (SSRs) markers for rapid identification of C. purpurea. SSRs were designed from sequence data stored at the National Center for Biotechnology Information database. The study consisted of 74 ergot isolates, from four different host species, Lolium perenne, Poa pratensis, Bromus inermis, and Secale cereale plus three additional Claviceps species, C. pusilla, C. paspali and C.fusiformis. Samples were collected from six different counties in Oregon and Washington over a 5-year period. Thirty-four SSR markers were selected, which enabled the differentiation of each isolate from one another based solely on their molecular fingerprints. Discriminant analysis of principle components was used to identify four isolate groups, CA Group 1, 2, 3, and 4, for subsequent cluster and molecular variance analyses. CA Group 1 consisting of eight isolates from the host species P. pratensis, was separated on the cluster analysis plot from the remaining three groups and this group was later identified as C. humidiphila. The other three groups were distinct from one another, but closely related. These three groups contained samples from all four of the host species. These SSRs are simple to use, reliable and allowed clear differentiation of C. humidiphila from C. purpurea. Isolates from the three separate species, C. pusilla, C. paspali and C.fusiformis, also amplified with these markers.The SSR markers developed in this study will be helpful in defining the population structure and genetics of Claviceps strains. They will also provide valuable tools for plant breeders needing to identify resistance in crops or for researchers examining fungal movements across environments.

Dataset Information

Classification of Southeast Asian mints (Mentha spp.) based on simple sequence repeat markers.

Publications

Classification of Southeast Asian mints (<i>Mentha</i> spp.) based on simple sequence repeat markers.

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