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Rapid conjugation of nanoparticles, proteins and siRNAs to microbubbles by strain-promoted click chemistry for ultrasound imaging and drug delivery.

ABSTRACT: A new strategy using catalyst-free strain-promoted alkyne-azide cycloaddition (SPAAC) "click" chemistry for the ligation of anti-cancer drug-loaded nanoparticles, functionalized proteins, and siRNA conjugated micelles to microbubbles (MB) was established. The results showed fast ligation within 5 min without sacrificing microbubble size and density. The ultrasound test showed good imaging abilities of the microbubbles after functionalization. This microbubble-therapeutic SPAAC "click" conjugation developed in the current study involves no toxic catalyst or initiator, has ultra-fast reaction speed, and is versatile for the ligation of various anti-cancer or therapeutic agents to microbubbles. These advantages render the SPAAC click strategy promising for broad applications in ultrasound-guided imaging and therapeutic delivery.

SUBMITTER: Liu X

PROVIDER: S-EPMC9523532 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Rapid conjugation of nanoparticles, proteins and siRNAs to microbubbles by strain-promoted click chemistry for ultrasound imaging and drug delivery.

Liu Xifeng X Gong Ping P Song Pengfei P Xie Feng F Miller A Lee AL Chen Shigao S Lu Lichun L

Polymer chemistry 20181227 6

A new strategy using catalyst-free strain-promoted alkyne-azide cycloaddition (SPAAC) "click" chemistry for the ligation of anti-cancer drug-loaded nanoparticles, functionalized proteins, and siRNA conjugated micelles to microbubbles (MB) was established. The results showed fast ligation within 5 min without sacrificing microbubble size and density. The ultrasound test showed good imaging abilities of the microbubbles after functionalization. This microbubble-therapeutic SPAAC "click" conjugatio ...[more]

PMID: 36187167

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