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ABSTRACT:
SUBMITTER: Woessmann J
PROVIDER: S-EPMC9552229 | biostudies-literature | 2022 Oct
REPOSITORIES: biostudies-literature
Woessmann Jakob J Kotol David D Hober Andreas A Uhlén Mathias M Edfors Fredrik F
Journal of proteome research 20220831 10
Protein quantification strategies using multiple proteases have been shown to deliver poor interprotease accuracy in label-free mass spectrometry experiments. By utilizing six different proteases with different cleavage sites, this study explores the protease bias and its effect on accuracy and precision by using recombinant protein standards. We established 557 SRM assays, using a recombinant protein standard resource, toward 10 proteins in human plasma and determined their concentration with m ...[more]