Comparative transcriptomics in human COPD reveals dysregulated genes uniquely expressed in ferrets.

ABSTRACT:

Background

Chronic obstructive pulmonary disease (COPD) is a progressive lung disease with poor treatment options. However, most mouse models of COPD produce a primarily emphysematous disease not recapitulating clinically meaningful COPD features like chronic bronchitis.

Methods

Wild-type ferrets (Mustela putorius furo) were divided randomly into two groups: whole body cigarette smoke exposure and air controls. Ferrets were exposed to smoke from 1R6F research cigarettes, twice daily for six months. RNA-sequencing was performed on RNA isolated from lung tissue. Comparative transcriptomics analyses of COPD in ferrets, mice, and humans were done to find the uniquely expressed genes. Further, Real-time PCR was performed to confirmed RNA-Seq data on multiple selected genes.

Re

SUBMITTER: Hussain SS 

PROVIDER: S-EPMC9552453 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature