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Noncoupled NADH:ubiquinone oxidoreductase of Azotobacter vinelandii is required for diazotrophic growth at high oxygen concentrations.

ABSTRACT: The gene encoding the noncoupled NADH:ubiquinone oxidoreductase (NDH II) from Azotobacter vinelandii was cloned, sequenced, and used to construct an NDH II-deficient mutant strain. Compared to the wild type, this strain showed a marked decrease in respiratory activity. It was unable to grow diazotrophically at high aeration, while it was fully capable of growth at low aeration or in the presence of NH(4)(+). This result suggests that the role of NDH II is as a vital component of the respiratory protection mechanism of the nitrogenase complex in A. vinelandii. It was also found that the oxidation of NADPH in the A. vinelandii respiratory chain is catalyzed solely by NDH II.

SUBMITTER: Bertsova YV 

PROVIDER: S-EPMC95528 | biostudies-literature | 2001 Dec

REPOSITORIES: biostudies-literature

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Process for packaging and dispensing frozen desserts

Levy Jérôme   Ferrari-Philippe Fabiana   Lanoy Thierry  

20020919

Iced dessert packaging and dispensing procedure uses container with dispenser and different gases to expand and dispense product The procedure, in which a container (1) has a propellant gas to dispense the product, uses a gas that is insoluble in the product to dispense it and a different gas to expand the product as it is dispensed. The expanding gas is preferably selected from nitrogen protoxide and carbon dioxide, and is dissolved in the frozen dessert product. The propellant gas is diazote o  ...[more]