Protocol for renal ischemia-reperfusion injury by flank incisions in mice.
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ABSTRACT:
SUBMITTER: Cheng YT
PROVIDER: S-EPMC9562430 | biostudies-literature | 2022 Oct
REPOSITORIES: biostudies-literature
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Protocol for renal ischemia-reperfusion injury by flank incisions in mice.
Cheng Yung-Ting YT Tu Ya-Chun YC Chou Yu-Hsiang YH Lai Chun-Fu CF
STAR protocols 20221007 4
Ischemia-reperfusion injury (IRI) contributes to acute kidney injury (AKI) and development of chronic kidney disease. We describe an IRI protocol for mice via flank incisions approach, using a pedicle clamp to cause ischemic injury. Compared with trans-abdominal approach, it is technically easier with lesser fluid loss and organ injury. Technical challenges during the dissection of renal pedicles are highlighted. For complete details on the execution of this protocol, please refer to Lai et al. ...[more]
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