Ontology highlight
ABSTRACT:
SUBMITTER: Kwak YD
PROVIDER: S-EPMC95715 | biostudies-literature | 1999 Jul
REPOSITORIES: biostudies-literature
Kwak Y D YD Yoo S K SK Kim E J EJ
Clinical and diagnostic laboratory immunology 19990701 4
A new system designed for cell surface display of recombinant proteins on Escherichia coli has been evaluated for expression of eukaryotic viral proteins. Human immunodeficiency virus type 1 (HIV-1) gp120 was fused to the C terminus of ice nucleation protein (INP), an outer membrane protein of Pseudomonas syringae. Western blotting, immunofluorescence microscopy, fluorescence-activated cell-sorting analysis, whole-cell enzyme-linked immunosorbent assay, and ice nucleation activity assay confirme ...[more]