Ontology highlight
ABSTRACT:
SUBMITTER: Vemulapalli R
PROVIDER: S-EPMC95834 | biostudies-literature | 2000 Jan
REPOSITORIES: biostudies-literature
Vemulapalli R R Cravero S S Calvert C L CL Toth T E TE Sriranganathan N N Boyle S M SM Rossetti O L OL Schurig G G GG
Clinical and diagnostic laboratory immunology 20000101 1
Using the shuttle vector pMCO2 and the vaccinia virus wild-type WR strain, we constructed a recombinant virus expressing an 18-kDa outer membrane protein of Brucella abortus. BALB/c mice inoculated with this virus produced 18-kDa protein-specific antibodies, mostly of immunoglobulin G2a isotype, and in vitro stimulation of splenocytes from these mice with purified maltose binding protein-18-kDa protein fusion resulted in lymphocyte proliferation and gamma interferon production. However, these mi ...[more]