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Clonal analysis of the human B-cell repertoire using a heteromobility assay.

ABSTRACT: A heteromobility duplex tracking assay was developed to analyze B-cell clonality. The assay was based on the genetic variability of B-cell immunoglobulin (Ig) sequences. Binding of amplified (Ig) sequences to a single-stranded radiolabeled Ig DNA probe resulted in the formation of heteroduplexes. The mobilities of these heteroduplexes helped to distinguish clonal B cells.

SUBMITTER: Tewari D

PROVIDER: S-EPMC95903 | biostudies-literature | 2000 May

REPOSITORIES: biostudies-literature

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Clonal analysis of the human B-cell repertoire using a heteromobility assay.

Tewari D D

Clinical and diagnostic laboratory immunology 20000501 3

A heteromobility duplex tracking assay was developed to analyze B-cell clonality. The assay was based on the genetic variability of B-cell immunoglobulin (Ig) sequences. Binding of amplified (Ig) sequences to a single-stranded radiolabeled Ig DNA probe resulted in the formation of heteroduplexes. The mobilities of these heteroduplexes helped to distinguish clonal B cells. ...[more]

PMID: 10799470

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