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Quantifying biofilm propagation on chemically modified surfaces.

ABSTRACT: Conditions affecting biofilm formation differ among bacterial species and this presents a challenge to studying biofilms in the lab. This work leverages functionalized silanes to control surface chemistry in the study of early biofilm propagation, quantified with a semi-automated image processing algorithm. These methods support the study of Pantoea sp. YR343, a gram-negative bacterium isolated from the poplar rhizosphere. We found that Pantoea sp. YR343 does not readily attach to hydrophilic surfaces but will form biofilms with a "honeycomb" morphology on hydrophobic surfaces. Our image processing algorithm described here quantified the evolution of the honeycomb morphology over time, and found the propagation to display a logarithmic behavior. This methodology was repeated with a flagella-deficient fliR mutant of Pantoea sp. YR343 which resulted in reduced surface attachment. Quantifiable differences between Pantoea WT and ΔfliR biofilm morphologies were captured by the image processing algorithm, further demonstrating the insight gained from these methods.

SUBMITTER: Halsted MC

PROVIDER: S-EPMC9594113 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Quantifying biofilm propagation on chemically modified surfaces.

Halsted Michelle C MC Bible Amber N AN Morrell-Falvey Jennifer L JL Retterer Scott T ST

Biofilm 20221015

Conditions affecting biofilm formation differ among bacterial species and this presents a challenge to studying biofilms in the lab. This work leverages functionalized silanes to control surface chemistry in the study of early biofilm propagation, quantified with a semi-automated image processing algorithm. These methods support the study of <i>Pantoea</i> sp. YR343, a gram-negative bacterium isolated from the poplar rhizosphere. We found that <i>Pantoea</i> sp. YR343 does not readily attach to ...[more]

PMID: 36303845

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Dataset Information

Quantifying biofilm propagation on chemically modified surfaces.

Publications

Quantifying biofilm propagation on chemically modified surfaces.

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