Microbiota DNA isolation, 16S rRNA amplicon sequencing, and bioinformatic analysis for bacterial microbiome profiling of rodent fecal samples.
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ABSTRACT:
SUBMITTER: Love CJ
PROVIDER: S-EPMC9597187 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
Publications
Microbiota DNA isolation, 16S rRNA amplicon sequencing, and bioinformatic analysis for bacterial microbiome profiling of rodent fecal samples.
Love Chloe J CJ Gubert Carolina C Kodikara Saritha S Kong Geraldine G Lê Cao Kim-Anh KA Hannan Anthony J AJ
STAR protocols 20221021 4
Fecal samples are frequently used to characterize bacterial populations of the gastrointestinal tract. A protocol is provided to profile gut bacterial populations using rodent fecal samples. We describe the optimal procedures for collecting rodent fecal samples, isolating genomic DNA, 16S rRNA gene V4 region sequencing, and bioinformatic analyses. This protocol includes detailed instructions and example outputs to ensure accurate, reproducible results and data visualization. Comprehensive troubl ...[more]
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