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Antimicrobial Activity of Apidermin 2 from the Honeybee Apis mellifera.


ABSTRACT: Apidermins (APDs) are known as structural cuticular proteins in insects, but their additional roles are poorly understood. In this study, we characterized the honeybee, Apis mellifera, APD 2 (AmAPD 2), which displays activity suggesting antimicrobial properties. In A. mellifera worker bees, the AmAPD 2 gene is transcribed in the epidermis, hypopharyngeal glands, and fat body, and induced upon microbial ingestion. Particularly in the epidermis of A. mellifera worker bees, the AmAPD 2 gene showed high expression and responded strongly to microbial challenge. Using a recombinant AmAPD 2 peptide, which was produced in baculovirus-infected insect cells, we showed that AmAPD 2 is heat-stable and binds to live bacteria and fungi as well as carbohydrates of microbial cell wall molecules. This binding action ultimately induced structural damage to microbial cell walls, which resulted in microbicidal activity. These findings demonstrate the antimicrobial role of AmAPD 2 in honeybees.

SUBMITTER: Kim BY 

PROVIDER: S-EPMC9604307 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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Antimicrobial Activity of Apidermin 2 from the Honeybee <i>Apis mellifera</i>.

Kim Bo-Yeon BY   Kim Yun-Hui YH   Choi Yong-Soo YS   Lee Man-Young MY   Lee Kwang-Sik KS   Jin Byung-Rae BR  

Insects 20221020 10


Apidermins (APDs) are known as structural cuticular proteins in insects, but their additional roles are poorly understood. In this study, we characterized the honeybee, <i>Apis mellifera</i>, APD 2 (AmAPD 2), which displays activity suggesting antimicrobial properties. In <i>A. mellifera</i> worker bees, the <i>AmAPD 2</i> gene is transcribed in the epidermis, hypopharyngeal glands, and fat body, and induced upon microbial ingestion. Particularly in the epidermis of <i>A. mellifera</i> worker be  ...[more]

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