Dataset Information

Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behcet's disease.

ABSTRACT:

Background

Macrophages are key innate immune cells implicated in the pathogenesis of Behçet's disease (BD), and macrophage polarization plays a pivotal role in inflammatory response. This study aimed to investigate the role of BD serum on the phenotypes and functions of macrophage polarization.

Methods

BD or HC serum-treated human monocyte-derived macrophages (HMDMs) were examined M1/M2 phenotypes using flow cytometry and ELISA. The phagocytic capacity of HMDMs and CD4⁺T cell differentiation facilitated by HMDMs were measured by flow cytometry. Transcriptome analysis of BD and HC serum-stimulated HMDMs was conducted to identify differentially expressed genes. NF-κB signaling was examined using western blot to explore the mechanism of macrophage polarization induced by BD serum.

Results

BD serum-treated macrophages expressed a higher level of CD86, IL-12, and TNF-α and a lower level of CD163, which were compatible with the M1-like phenotype. Furthermore, BD serum-treated macrophages showed enhanced phagocytic capacity and promoted more Th1 cell differentiation. Sixty-one differentially expressed genes were identified between BD and HC serum-treated macrophages and were enriched in NF-κB signaling. BD serum-treated macrophages showed upregulated p-p65 and downregulated IκBα, and NF-κB inhibitor attenuated BD serum-stimulated M1-like phenotype.

Conclusions

BD serum promoted macrophage polarization toward a proinflammatory M1-like phenotype through NF-κB signaling and potentially facilitated inflammation in BD. M1 polarized macrophages may be a potential therapeutic target for BD.

SUBMITTER: Wu X

PROVIDER: S-EPMC9635113 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Publications

Macrophage polarization toward M1 phenotype through NF-κB signaling in patients with Behçet's disease.

Wu Xiuhua X Wang Zhimian Z Shi Jing J Yu Xin X Li Chaoran C Liu Jinjing J Zhang Fengchun F Chen Hua H Zheng Wenjie W

Arthritis research & therapy 20221104 1

<h4>Background</h4>Macrophages are key innate immune cells implicated in the pathogenesis of Behçet's disease (BD), and macrophage polarization plays a pivotal role in inflammatory response. This study aimed to investigate the role of BD serum on the phenotypes and functions of macrophage polarization.<h4>Methods</h4>BD or HC serum-treated human monocyte-derived macrophages (HMDMs) were examined M1/M2 phenotypes using flow cytometry and ELISA. The phagocytic capacity of HMDMs and CD4<sup>+</sup> ...[more]

PMID: 36333776

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