Dataset Information

Estimation of skeletal kinematics in freely moving rodents.

ABSTRACT: Forming a complete picture of the relationship between neural activity and skeletal kinematics requires quantification of skeletal joint biomechanics during free behavior; however, without detailed knowledge of the underlying skeletal motion, inferring limb kinematics using surface-tracking approaches is difficult, especially for animals where the relationship between the surface and underlying skeleton changes during motion. Here we developed a videography-based method enabling detailed three-dimensional kinematic quantification of an anatomically defined skeleton in untethered freely behaving rats and mice. This skeleton-based model was constrained using anatomical principles and joint motion limits and provided skeletal pose estimates for a range of body sizes, even when limbs were occluded. Model-inferred limb positions and joint kinematics during gait and gap-crossing behaviors were verified by direct measurement of either limb placement or limb kinematics using inertial measurement units. Together we show that complex decision-making behaviors can be accurately reconstructed at the level of skeletal kinematics using our anatomically constrained model.

SUBMITTER: Monsees A

PROVIDER: S-EPMC9636019 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Estimation of skeletal kinematics in freely moving rodents.

Monsees Arne A Voit Kay-Michael KM Wallace Damian J DJ Sawinski Juergen J Charyasz Edyta E Scheffler Klaus K Macke Jakob H JH Kerr Jason N D JND

Nature methods 20221017 11

Forming a complete picture of the relationship between neural activity and skeletal kinematics requires quantification of skeletal joint biomechanics during free behavior; however, without detailed knowledge of the underlying skeletal motion, inferring limb kinematics using surface-tracking approaches is difficult, especially for animals where the relationship between the surface and underlying skeleton changes during motion. Here we developed a videography-based method enabling detailed three-d ...[more]

PMID: 36253644

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Dataset Information

Estimation of skeletal kinematics in freely moving rodents.

Publications

Estimation of skeletal kinematics in freely moving rodents.

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