MiR-204-3p downregulates KRT16 and promotes corneal repair in tree shrew fungal keratitis model.

ABSTRACT:

Objectives

Corneal repair is critical for the treatment and recovery of corneal injuries. However, the molecular mechanism underlying corneal repair remains unclear.

Methods

A tree shrew model of corneal fungal infection was established by injecting Fusarium solani into the corneal stroma to study the role of miR-204-3p in repairing corneal injury induced by fungal keratitis and to explore the potential mechanisms underlying the repair process.

Results

miR-204-3p expression was significantly downregulated, while KRT16 expression was significantly upregulated after F. solani infection in the cornea of tree shrews. Moreover, miR-204-3p injection promoted corneal injury repair post-infection, potentially by downregulating KRT16 expression. Results of a luciferase reporter gene assay showed that miR-204-3p had a targeted relationship with KRT16. KRT16 protein expression levels decreased after miR-204-3p injection into the cornea with fungal keratitis, reducing the degree of corneal injury.

Conclusions

In this study, we report for the first time that miR-204-3p and KRT16 influence the repair of corneal injury. In addition, their effects on the repair of corneal injury were studied in a tree shrew model, providing an experimental basis for the study of pathogenesis of human fungal keratitis.

SUBMITTER: Jia J 

PROVIDER: S-EPMC9641470 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature