Dataset Information

Characterization of an Entner-Doudoroff pathway-activated Escherichia coli.

ABSTRACT:

Background

Escherichia coli have both the Embden-Meyerhof-Parnas pathway (EMPP) and Entner-Doudoroff pathway (EDP) for glucose breakdown, while the EDP primarily remains inactive for glucose metabolism. However, EDP is a more favorable route than EMPP for the production of certain products.

Results

EDP was activated by deleting the pfkAB genes in conjunction with subsequent adaptive laboratory evolution (ALE). The evolved strains acquired mutations in transcriptional regulatory genes for glycolytic process (crp, galR, and gntR) and in glycolysis-related genes (gnd, ptsG, and talB). The genotypic, transcriptomic and phenotypic analyses of those mutations deepen our understanding of their beneficial effects on cellulosic biomass bio-conversion. On top of these scientific understandings, we further engineered the strain to produce higher level of lycopene and 3-hydroxypropionic acid.

Conclusions

These results indicate that the E. coli strain has innate capability to use EDP in lieu of EMPP for glucose metabolism, and this versatility can be harnessed to further engineer E. coli for specific biotechnological applications.

SUBMITTER: Kim YE

PROVIDER: S-EPMC9648032 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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Publications

Characterization of an Entner-Doudoroff pathway-activated Escherichia coli.

Kim Ye Eun YE Cho Kyung Hyun KH Bang Ina I Kim Chang Hee CH Ryu Young Shin YS Kim Yuchan Y Choi Eun Mi EM Nong Linh Khanh LK Kim Donghyuk D Lee Sung Kuk SK

Biotechnology for biofuels and bioproducts 20221109 1

<h4>Background</h4>Escherichia coli have both the Embden-Meyerhof-Parnas pathway (EMPP) and Entner-Doudoroff pathway (EDP) for glucose breakdown, while the EDP primarily remains inactive for glucose metabolism. However, EDP is a more favorable route than EMPP for the production of certain products.<h4>Results</h4>EDP was activated by deleting the pfkAB genes in conjunction with subsequent adaptive laboratory evolution (ALE). The evolved strains acquired mutations in transcriptional regulatory ge ...[more]

PMID: 36352474

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Dataset Information

Characterization of an Entner-Doudoroff pathway-activated Escherichia coli.

Background

Results

Conclusions

Publications

Characterization of an Entner-Doudoroff pathway-activated Escherichia coli.

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