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At the molecular resolution with MINFLUX?

ABSTRACT: MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1-3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the performance with other super-resolution methods focusing particularly on spatial resolution claims, subjective filtering of localizations, detection versus labelling efficiency and the possible limitations when imaging biological samples containing densely labelled structures. I hope the analysis and evaluation parameters presented here are not only useful for future research directions for single-molecule techniques but also microscope users, developers and core facility managers when deciding on an investment for the next 'state-of-the-art' instrument. This article is part of the Theo Murphy meeting issue 'Super-resolution structured illumination microscopy (part 2)'.

SUBMITTER: Prakash K

PROVIDER: S-EPMC9653251 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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At the molecular resolution with MINFLUX?

Prakash Kirti K

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences 20220214 2220

MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1-3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the performance with other super-resolution methods focusing particularly on spatial resolution claims, subjective filtering of localizations, detection versus labelling efficiency and the possible limita ...[more]

PMID: 35152756

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