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Genome-wide profiling of histone modifications in Plasmodium falciparum using CUT&RUN.

ABSTRACT: We recently adapted a CUT&RUN protocol for genome-wide profiling of chromatin modifications in the human malaria parasite Plasmodium Using the step-by-step protocol described below, we were able to generate high-quality profiles of multiple histone modifications using only a small fraction of the cells required for ChIP-seq. Using antibodies against two commonly profiled histone modifications, H3K4me3 and H3K9me3, we show here that CUT&RUN profiling is highly reproducible and closely recapitulates previously published ChIP-seq-based abundance profiles of histone marks. Finally, we show that CUT&RUN requires substantially lower sequencing coverage for accurate profiling compared with ChIP-seq.

SUBMITTER: Morillo RC

PROVIDER: S-EPMC9670794 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature

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Genome-wide profiling of histone modifications in Plasmodium falciparum using CUT&RUN.

Morillo Riward Campelo RC Harris Chantal T CT Kennedy Kit K Henning Samuel R SR Kafsack Björn Fc BF

Life science alliance 20221115 1

We recently adapted a CUT&RUN protocol for genome-wide profiling of chromatin modifications in the human malaria parasite Plasmodium Using the step-by-step protocol described below, we were able to generate high-quality profiles of multiple histone modifications using only a small fraction of the cells required for ChIP-seq. Using antibodies against two commonly profiled histone modifications, H3K4me3 and H3K9me3, we show here that CUT&RUN profiling is highly reproducible and closely reca ...[more]

PMID: 36379668

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