Unknown

Dataset Information

0

H2A.Z deposition by SWR1C involves multiple ATP-dependent steps.

ABSTRACT: Histone variant H2A.Z is a conserved feature of nucleosomes flanking protein-coding genes. Deposition of H2A.Z requires ATP-dependent replacement of nucleosomal H2A by a chromatin remodeler related to the multi-subunit enzyme, yeast SWR1C. How these enzymes use ATP to promote this nucleosome editing reaction remains unclear. Here we use single-molecule and ensemble methodologies to identify three ATP-dependent phases in the H2A.Z deposition reaction. Real-time analysis of single nucleosome remodeling events reveals an initial priming step that occurs after ATP addition that involves a combination of both transient DNA unwrapping from the nucleosome and histone octamer deformations. Priming is followed by rapid loss of histone H2A, which is subsequently released from the H2A.Z nucleosomal product. Surprisingly, rates of both priming and the release of the H2A/H2B dimer are sensitive to ATP concentration. This complex reaction pathway provides multiple opportunities to regulate timely and accurate deposition of H2A.Z at key genomic locations.

SUBMITTER: Fan J 

PROVIDER: S-EPMC9672302 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

H2A.Z deposition by SWR1C involves multiple ATP-dependent steps.

Fan Jiayi J   Moreno Andrew T AT   Baier Alexander S AS   Loparo Joseph J JJ   Peterson Craig L CL  

Nature communications 20221117 1

Histone variant H2A.Z is a conserved feature of nucleosomes flanking protein-coding genes. Deposition of H2A.Z requires ATP-dependent replacement of nucleosomal H2A by a chromatin remodeler related to the multi-subunit enzyme, yeast SWR1C. How these enzymes use ATP to promote this nucleosome editing reaction remains unclear. Here we use single-molecule and ensemble methodologies to identify three ATP-dependent phases in the H2A.Z deposition reaction. Real-time analysis of single nucleosome remod  ...[more]

Similar Datasets

Unknown Chromatin remodeling by imitation switch (ISWI) class ATP-dependent remodelers is stimulated by histone variant H2A.Z.
| S-EPMC2836070 | biostudies-literature
Unknown ?Np63? represses anti-proliferative genes via H2A.Z deposition.
| S-EPMC3475804 | biostudies-literature
Unknown Redundant Functions for Nap1 and Chz1 in H2A.Z Deposition.
| S-EPMC5589762 | biostudies-literature
Unknown Histone variants H2A.Z and H3.3 coordinately regulate PRC2-dependent H3K27me3 deposition and gene expression regulation in mES cells.
| S-EPMC6151936 | biostudies-literature
Unknown Deposition of histone variant H2A.Z within gene bodies regulates responsive genes.
| S-EPMC3469445 | biostudies-literature
Unknown H3K56 deacetylation and H2A.Z deposition are required for aberrant heterochromatin spreading.
| S-EPMC9023284 | biostudies-literature
Unknown pH-dependent 11° F1FO ATP synthase sub-steps reveal insight into the FO torque generating mechanism.
| S-EPMC8754430 | biostudies-literature
Unknown Histone variant H2A.Z deposition and acetylation directs the canonical Notch signaling response.
| S-EPMC6144792 | biostudies-literature
Unknown VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis.
| S-EPMC7408173 | biostudies-literature
Unknown ATP-dependent steps in the binding of ubiquitin conjugates to the 26S proteasome that commit to degradation.
| S-EPMC3038635 | biostudies-literature