Leveraging a natural murine meiotic drive to suppress invasive populations.

ABSTRACT: Invasive rodents are a major cause of environmental damage and biodiversity loss, particularly on islands. Unlike insects, genetic biocontrol strategies including population-suppressing gene drives with biased inheritance have not been developed in mice. Here, we demonstrate a gene drive strategy (t_CRISPR) that leverages super-Mendelian transmission of the t haplotype to spread inactivating mutations in a haplosufficient female fertility gene (Prl). Using spatially explicit individual-based in silico modeling, we show that t_CRISPR can eradicate island populations under a range of realistic field-based parameter values. We also engineer transgenic t_CRISPR mice that, crucially, exhibit biased transmission of the modified t haplotype and Prl mutations at levels our modeling predicts would be sufficient for eradication. This is an example of a feasible gene drive system for invasive alien rodent population control.

SUBMITTER: Gierus L 

PROVIDER: S-EPMC9674240 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature