Dataset Information

Expression and regulatory characteristics of peripheral blood immune cells in primary Sjogren's syndrome patients using single-cell transcriptomic.

ABSTRACT: Immune cell subgroups in peripheral blood mononuclear cells (PBMCs) in primary Sjogren's syndrome (pSS) are thought to regulate immune responses, but the nature and functions of these subgroups remain unclear. Here we performed single-cell RNA sequencing (scRNA-seq) of about 68,500 PBMCs from three patients with pSS and three healthy controls (HCs). We found that CD14⁺ monocytes from pSS patients expressed high levels of the transcription factor CEBPD, and the direct regulation of target genes expression by CEBPD tends to participate in the TNF-α signaling via NF-κB in monocytes. FOLR3 and IL1B were upregulated separately in CD14⁺ monocyte subsets from different pSS patients. We proposed a system for classifying CD56^-CD16⁺ NK cells based on FCER1G expression. Compared with HCs, pSS patients showed a significantly higher ratio of CD56^-CD16+FCER1G+ NK cells to CD56^-CD16+FCER1G- NK cells. Our analysis provides a reference dataset and reveals its immune heterogeneity among PBMCs in pSS.

SUBMITTER: Liu J

PROVIDER: S-EPMC9678742 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Expression and regulatory characteristics of peripheral blood immune cells in primary Sjögren's syndrome patients using single-cell transcriptomic.

Liu Jinkun J Gao Hongyan H Li Chengyin C Zhu Fenglin F Wang Miao M Xu Yanqiu Y Wu Bin B

iScience 20221104 12

Immune cell subgroups in peripheral blood mononuclear cells (PBMCs) in primary Sjogren's syndrome (pSS) are thought to regulate immune responses, but the nature and functions of these subgroups remain unclear. Here we performed single-cell RNA sequencing (scRNA-seq) of about 68,500 PBMCs from three patients with pSS and three healthy controls (HCs). We found that CD14<sup>+</sup> monocytes from pSS patients expressed high levels of the transcription factor <i>CEBPD</i>, and the direct regulation ...[more]

PMID: 36425764

Similar Datasets

Project description:ObjectivePrimary Sjögren's syndrome (pSS) is a systemic autoimmune disorder with an unclear pathogenetic mechanism in the labial gland. This study aims to investigate the cellular and molecular mechanisms contributing to the development of this disease.MethodsSingle-cell RNA sequencing (scRNA-seq) was performed on 32,337 cells of labial glands from three pSS patients and three healthy individuals. We analyzed all cell subsets implicated in pSS pathogenesis.ResultsOur research revealed diminished differentiation among epithelial cells, concomitant with an enhancement of interferons (IFNs)-mediated signaling pathways. This indicates a cellular functional shift in reaction to inflammatory triggers. Moreover, we observed an augmentation in the population of myofibroblasts and endothelial cells, likely due to the intensified IFNs signaling, suggesting a possible reconfiguration of tissue structure and vascular networks in the impacted regions. Within the immune landscape, there was an apparent increase in immunosuppressive macrophages and dendritic cells (DCs), pointing to an adaptive immune mechanism aimed at modulating inflammation and averting excessive tissue harm. Elevated activation levels of CD4+T cells, along with a rise in regulatory T (Treg) cells, were noted, indicating a nuanced immune interplay designed to manage the inflammatory response. In the CD8+T cell subsests, we detected a notable increase in cells expressing granzyme K (GZMK), signaling an intensified cytotoxic activity. Additionally, the escalated presence of T cells with high levels of heat shock proteins (HSPs) suggests a cellular stress condition, possibly associated with persistent low-grade inflammation, mirroring the chronic aspect of the condition.ConclusionsOur research identified distinct stromal and immune cell populations linked to pSS, revealing new potential targets for its management. The activation of myeloid, B, and T cells could contribute to pSS pathogenesis, providing important guidance for therapeutic approaches.

Dataset Information

Expression and regulatory characteristics of peripheral blood immune cells in primary Sjogren's syndrome patients using single-cell transcriptomic.

Publications

Expression and regulatory characteristics of peripheral blood immune cells in primary Sjögren's syndrome patients using single-cell transcriptomic.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets